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KMID : 0364820020380020103
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Korean Journal of Microbiology
2002 Volume.38 No. 2 p.103 ~ p.106
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Optimization of PCR Conditin with Conidiospore for Primary Screening of Aspergillus nidulans Transformants
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Abstract
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irect PCR form intact fungal cells is not readily suitable to all fungi mainly because of difficulties in rupturing the cell walls. Microwave irradiation has been proven to be useful in fungal DNA extraction protocol. Here we describe a fast template preparation method for PCR amplification from Aspergillus nidulans conidiospores using microwave irradiation. We optimized the duration for microwave irradiation, and the amount of template DNA for PCR. Amplification from samples prepared in this manner was so efficient that we could get PCR products with size enough to identify transformants. We believe that this is a time-saving procedure for screening true transformants of A. nidulans.
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KEYWORD
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conidiospore, Aspergillus nidulans, microwave irradiatin, PCR
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